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KMID : 0881720170320060447
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Journal of Food Hygiene and Safety
2017 Volume.32 No. 6 p.447 ~ p.454
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Detection of Food-Grade Hydrogen Peroxide by HRP-Biocomposite Modified Biosensors
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Chang Seung-Cheol
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Abstract
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A new amperometric biosensor has been developed for the detection of hydrogen peroxide (H2O2). The sensor was fabricated through the one-step deposition of a biocomposite layer onto a glassy carbon electrode at neutral pH. The biocomposite, as a H2O2 sensing element, was prepared by the electrochemical deposition of a homogeneous mixture of graphene oxide, aniline, and horseradish peroxidase. The experimental results clearly demonstrated of that the sensor possessed high electrocatalytic activity and responded to H2O2 with a stable and rapid manners. Scanning electron microscopy, cyclic voltammetry, and amperometry were performed to optimize the characteristics of the sensor and to evaluate its sensing chemistry. The sensor exhibited a linear response to H2O2 in the range of 10 to 500 μM concentrations, and its detection limit was calculated to be 1.3 μM. The proposed sensing-chemistry strategy and the sensor format were simple, cost-effective, and feasible for analysis of “food-grade H2O2” in food samples.
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KEYWORD
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Food-Grade Hydrogen Peroxide , Biosensor , Amperometry , Enzyme Immobilization
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